Hoʻāʻo ʻia ʻo Infectious Canine Hepatitis(ICH).

ʻO ka maʻi hepatitis canine (ICH) ma muli o canine adenovirus 1 (CAV-1) he maʻi lele o nā ʻīlio ma ka honua holoʻokoʻa, me nā hōʻailona lapaʻau e ʻokoʻa ana mai ke kuni liʻiliʻi a me ka hoʻopaʻa ʻana o nā membrane mucous a hiki i ke kaumaha nui, ka leukopenia i kaha ʻia, a me nā maʻi coagulation. . Hoʻopili pinepine nā hōʻailona maʻi a me nā mea ʻino ʻole o ke keʻena hana i ke ʻano o ka maʻi maʻi e hoʻoulu i ka necrosis hepatocellular a hoʻolaha i ka pōʻino endothelial cell; akā naʻe, hiki ke alakaʻi i ka maʻi uveitis, ka ʻāhu o ke kino, a me ka glomerulonephritis. Hiki ke hana ʻia ka ʻike ma ka hoʻohana ʻana i ka ELISA, serologic, a i ʻole PCR hoʻāʻo, a me nā diagnostics postmortem me ka ʻike ʻana i nā kino hoʻokomo intranuclear i loko o ke ake, PCR assay, a/a i ʻole FISH o ka ʻiʻo maʻi.

ʻAno laʻana:

Pono huahana:

Kiʻekiʻe ʻike pololei
ʻO ke kumukūʻai kiʻekiʻe
ʻO ka hōʻoia maikaʻi
Hoʻouna wikiwiki

E HOAKAKA ANO

Hoohana Manao

ʻO ka HG Rapid Test ICH kahi pahu immunoassay chromatographic no ka ʻike wikiwiki a me ka qualitative o nā antigens canine adenovirus 1(CAV1) i loko o ke koko, kahi e hoʻopiʻi ai i ka maʻi hepatitis canine (ICH). Hoʻokuʻu ʻia ka CAV1-specificantibody ma luna o ka membrane nitrocellulose. A ua hui pū ʻia kekahi CAV1 - nā antibodies kikoʻī i 40 nm o nā ʻāpana gula colloidal. Hoʻokomo ʻia kēia conjugate ma luna o ka polyester a i ʻole ka pā aniani e like me ka conjugate pad. Ke hoʻokuʻu ʻia ka hāpana i loko o ka hāpana maikaʻi ma ka hāmeʻa, neʻe ka conjugate solubilized me ka hāpana ma o ka hoʻopuehu passive a hui pū ka conjugate a me ka laʻana me nā antibodies i hoʻopaʻa ʻia i ka nitrocellulose. Inā loaʻa i ka hāpana nā antigens adenovirus, ʻike ʻia ka hopena i loko o 10 mau minuke ma ke ʻano o kahi laina ʻulaʻula e ulu ana ma ka membrane. Ke hoʻomau nei ka neʻe ʻana o ka hopena e hālāwai me kahi reagent mana, a laila e hana ana i kahi laina ʻulaʻula (laina mana).

Nā hōʻailona

1. Ka wela,

2. Lethargy,

3. Ka hemahema,

4. Ka luaʻi ʻana,

5.Hemorrhagic diarrhea,

6. ʻeha ʻōpū,

7. Dehydration,

8. Ka maʻi ʻeha,

9.Petechial hemorrhages,

10. ʻAha

Nā hiʻohiʻona

1 Hoʻohana kēia huahana i ka loina o ka immunochromatography gula colloidal e ʻike koke i ka ICH Antigen.

2 ʻAʻole pono kēia huahana i nā mea hana kūikawā, a hiki ke hoʻokō wikiwiki a maʻalahi hoʻi ma-ka nānā pūnaewele.

3 Me ka hoʻohana ʻana i nā mea maka maʻemaʻe kiʻekiʻe a me nā mea kiʻekiʻe-kiʻekiʻe, hōʻike ia i ka ʻike kiʻekiʻe a me ka kikoʻī.

Hana

E kau i nā mea hoʻohālike a pau, nā mea hoʻāʻo, a me ka hoʻonā Assay a ʻae iā lākou i ka lumi wela ma mua o ka hoʻāʻo ʻana (15 ~ 30 min).

E hoʻomākaukau i ka mea hoʻāʻo e like me kāu e pono ai, a laila e kaha i nā maʻi, ID ma luna o ka hāmeʻa, E ʻoluʻolu e hana i ka hoʻāʻo ma hope koke o ka wehe ʻana i ka hāmeʻa mai ka ʻeke pepa.

E hoʻouka i 1 kulu (10ul) o ka specimen i loko o ka pūnāwai hāpana (S) me ka paipu capillary. ʻO ka leo hāpana i ka laina kuhikuhi 10 μL.

E hoʻohui i 3 kulu o ka Assay Solution i loko o ka pūnāwai hāpana (S).

E wehewehe i ka hopena ho'āʻo i loko o 10 mau minuke. Mai heluhelu i ka hopena ma hope o 10 mau minuke.

Ka wehewehe ʻana i nā hopena

Kuhi ʻia kēia huahana e hoʻomaʻamaʻa i ke keʻena olakino olakino ma ka honua holoʻokoʻa e hoʻonui i ko lākou hiki ke hoʻopaʻa i ka wā e kū ana i ka pilikia olakino e hiki mai ana ʻo Infectious Canine Hepatitis, e ʻae ana i ka pane olakino olakino maikaʻi a wikiwiki hoʻi i ka hihia o kahi maʻi maʻi.