Ukusetyenziswa kwenjongo

I-HIV 1/2 Isixhobo soVavanyo lwe-HIV (igazi elipheleleyo / i-serum / i-plasma) yi-chidmoographic eguqukayo kwi-antibodies ye-antibodies kwi-HIV ye-HIV

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Imikhombandlela yokusetyenziswa

Vumela isixhobo sovavanyo, i-buffer, i-buffer, kunye / okanye ilawula ukuya

Ukulinganisa kubushushu begumbi (15 - 30 ° C) ngaphambi kovavanyo.

1. Susa isixhobo sovavanyo kwi-foil pouch kwaye ulisebenzise ngokukhawuleza. Iziphumo ezilungileyo ziya kufumaneka ukuba i-assay yenziwe ngaphakathi kwiyure enye.

2. Beka isixhobo sovavanyo kumgangatho ococekileyo kunye nenqanaba. I-SMUUM, i-PLAUM ye-plasma: Bamba i-Dropper ngokuthe nkqo kwaye ugqithisele i-Stoms 1 μm) kwi-charpimen yesixhobo sovavanyo, emva koko wongeza i-buffer (malunga ne-40 μ) kwaye uqalise ixesha. Jonga umfanekiso ongezantsi. Ukuthathwa kwegazi igazi: Bamba i-Dropper ngokuthe nkqo kwaye ugqithisele amaconsi ama-2 yokuqhubela phambili igazi (malunga ne-80 μl) kwi-timen yesixhobo se-2 uffer (malunga ne-40 μl) kwaye uqalise ixesha. Jonga umfanekiso ongezantsi. Kwiminwe yeminwe yonke yegazi: Vumela i-3 yokuxhoma iminwe yeminwe yonke (malunga ne-80 μl) ukuba iwele embindini wezinto zovavanyo, emva koko yongeza

I-2 yehla se-buffer (malunga ne-40 μl) kwaye uqalise ixesha. Jonga umfanekiso ongezantsi.

3. Lindela umgca obomvu (s) obonakalayo. Isiphumo kufuneka sifundwe kwimizuzu eli-15. Sukutolika iziphumo emva kwemizuzu engama-20.

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(Nceda ujonge kumzekeliso ongentla)

I-Honess: Iifizi ezimbini ezizodwa ziyavela. Umgca omnye kufuneka ube kwingingqi yolawulo (c) kwaye omnye umgca kufuneka abe kwingingqi yovavanyo (T).

*PHAWULA:Ubunzulu bombala obomvu kummandla wovavanyo (T) luya kwahluka ngokuxhomekeke kuxinzelelo lwee-antibodies ze-HIV ezikhoyo kwinqaku. Ke ngoko, nawuphi na umthunzi obomvu kummandla wovavanyo (T) kufuneka uthathwe ukuba ube nengqondo.

I-negating: umgca obomvu uvela kwingingqi yolawulo (c).Akukho mgca ubomvu obonakalayo okanye u-Pink uvela kummandla wovavanyo (T).

Ayisebenzi: Umgca wolawulo uyasilela ukuvela.Ivolumu engonelanga okanye iindlela zenkqubo ezingachanekanga zezona zizathu zibalulekileyo zokusilela komgca. Phonononga le nkqubo kwaye uphinda uvavanyo ngesixhobo esitsha sovavanyo. Ukuba ingxaki iyaqhubeka, ukuyeka ukusebenzisa ikhithi yovavanyo kwangoko kwaye uqhagamshelane nomsasazi wakho wengingqi.